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采集了我国沿海共计9个缢蛏(Sinonovacula constricta)地理群体的197个样本,分别是北部组群的3个群体:辽宁省庄河群体(ZH),天津市汉沽群体(HG),山东省海阳群体(HY);中部组群的3个群体:江苏省盐城群体(YC),上海市崇明县东滩群体(DT)和堡镇群体(BZ);以及南部组群的3个群体:浙江省宁波群体(NB),浙江省台州群体(TZ)以及福建省宁德群体(ND)。利用线粒体COⅠ标记分析了9个群体的遗传多样性和遗传分化。结果表明,在共计197个个体中检测到125个单倍型和96个变异位点,核苷酸多样性指数位于2.1764～7.4970之间,其中中部组群的群体遗传多样性指数最高。AMOVA分析结果显示,组间遗传变异量占总变异的80.27%,18.74%来自于群体内,只有0.99%来自于组内群体间。群体间遗传分化系数位于0.0219～0.8706之间,不同群体间具有一定的遗传分化,尤其是中部群体与其他群体间遗传分化值达到了0.8以上,为极高度分化。遗传距离和聚类结果显示,北部3群体和南部3群体首先聚在一起,之后与中部3群体聚类。
A total of 197 samples of nine geographical populations of Sinonovacula constricta along the coast of China were collected, including three groups from the northern group: Zhuanghe Group in Liaoning Province, HG in Tianjin, Haiyang in Shandong Province, (HY); three groups in the middle group: Yancheng Group (YC) in Jiangsu Province, Dongtan Group (DT) and BZ Group in Chongming County of Shanghai; and three groups in the southern group: Zhejiang Ningbo (NB), Taizhou, Zhejiang (TZ) and Ningde (Fujian Province). Nine populations were analyzed for genetic diversity and genetic differentiation using mitochondrial COI markers. The results showed that 125 haplotypes and 96 variation sites were detected in a total of 197 individuals. The nucleotide diversity index ranged from 2.1764 to 7.4970, of which the population genetic diversity index of the middle group was the highest. AMOVA analysis showed that the genetic variation among groups accounted for 80.27% of the total variance, 18.74% from the population, only 0.99% from the group within the group. The coefficient of genetic differentiation among populations ranged from 0.0219 to 0.8706, with some genetic differentiation among different populations. Especially, the genetic differentiation between the central population and other populations reached more than 0.8, which is extremely highly differentiated. Genetic distances and clustering results showed that 3 groups in the north and 3 groups in the south clustered together first, and then clustered with 3 groups in the middle.