论文部分内容阅读
为了致弱锦鲤疱疹病毒得到减毒株,试验采用细叶桉叶的提取液与病毒在细胞中共培养来致弱病毒,并以中和抗体试验检验弱毒株的免疫原性。结果表明:利用获得的减毒株在加强免疫42天时,抗锦鲤疱疹病毒中和抗体效价达到最高水平,同时灭活病毒免疫组的抗锦鲤疱疹病毒中和抗体水平低于KHV-P_(52)免疫组,PBS对照组未检测出抗锦鲤疱疹病毒的中和抗体,两个免疫组与对照组比较差异显著(P<0.05);KHV-P52免疫组对鲤鱼的免疫保护效果好,免疫保护率为100%。说明细叶桉叶提取液能有效致弱锦鲤疱疹病毒,通过试验获得了一株锦鲤疱疹病毒弱毒株。
In order to attenuate the koi herpes virus attenuated strain, the experiment using eucalyptus leaves extract and virus co-cultured in the cell to weak virus, and the neutralizing antibody test to test the virulence of the attenuated strain. The results showed that the titers of anti-Koi herpesvirus neutralizing antibody reached the highest level at 42 days after booster immunization, and the level of neutralizing antibody against Koi herpesvirus was lower than that of KHV-P_ (52) immunized group, PBS control group did not detect anti-Koi herpes virus neutralizing antibodies, the two immunized groups compared with the control group significant difference (P <0.05); KHV-P52 immunized group of carp immunoprotective effect is good , Immune protection rate was 100%. Description Eucalyptus leaves extract can effectively weaken the koi herpes virus, obtained through the test a koi herpes virus attenuated strains.