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为了掌握易捕鲤与亲本之间亲缘关系的偏移程度,试验采用聚丙烯酰胺凝胶电泳方法分析了易捕鲤及其选育亲本大头鲤、黑龙江鲤和散鳞镜鲤的肝脏和肌肉的淀粉酶(AMY)、酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、超氧化物歧化酶(SOD)、乙醇脱氢酶(ADH)6种同工酶。结果表明:4种鲤鱼各种同工酶带型基本一致,易捕鲤同工酶活性与亲本一致或者介于亲本之间,并且更接近大头鲤。说明易捕鲤的遗传背景与选育结果相同,没有发生较大偏移。
In order to grasp the deviation of the relationship between easy carp and their parents, polyacrylamide gel electrophoresis was used to analyze the liver and muscle of easy carp and its breeding parents Carp, Heilongjiang Carp and Scirpus mirror carp Amylase (AMY), esterase (EST), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), superoxide dismutase (SOD) and alcohol dehydrogenase (ADH) . The results showed that all isozymes belonged to the same kind of common carp, and the isozyme activity of common carp was the same as that of the parent or between parents, and closer to the big carp. The results showed that the genetic background of easy-to-catch carp was the same as that of breeding, and no big deviation occurred.