环一磷腺苷(以下简称cAMP)作为细胞内介质,能介导多种激素的作用,所以称为第二信使,这已是众所周知的事实。但cAMP对细胞生长的影响,直到1968年才由Burk和Ryan分别报导。由多瘤病毒或罗斯肉瘤病毒转化的BHK细胞培养内,加入茶硷或咖啡因后,在指数期细胞生长减慢,Burk认为这是由于磷酸二酯酶被抑制,细胞内cAMP增高的结果。Ryan发现HeLa细胞和L细胞株在cAMP影响下,细胞增殖受抑制。从 Cyclic adenosine monophosphate (hereinafter referred to as cAMP) acts as an intracellular medium and can mediate the action of various hormones. Therefore, it is known as a second messenger. This is a well-known fact. However, the effect of cAMP on cell growth was not reported by Burk and Ryan until 1968. In BHK cell cultures transformed with polyomavirus or Rose sarcoma virus, the growth of cells in the exponential phase is slowed down after the addition of saponin or caffeine, which Burk believes is due to the inhibition of phosphodiesterase and increased intracellular cAMP. Ryan found that HeLa cells and L cell lines are inhibited by cAMP. From