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目的研究开封地区2008年病毒性脑炎病原分离株Echo30VP1区基因特征及其分子流行病学特点。方法对开封地区2008年病毒性脑炎监测病例脑脊液标本中分离到的8株Echo30病毒进行VP1区全基因序列测定,与GeneBank上发表的ECHO30型VP1区进行同源性比较,通过构建进化树对其进行遗传进化分析。结果所测8株Echo30VP1区基因全长均为876bp,翻译的氨基酸全长292aa。8株病毒VP1区核苷酸同源性为92.8%～99.7%,氨基酸同源性为93.0%～99.7%;与GenBank相关毒株基因组序列比对显示开封地区2008年分离株均与江苏省2003年分离株同源性较高;进化分析表明属于第7组。结论 2008年开封地区Echo30分离株与2003年江苏省分离株亲缘关系最近,可能具有相同来源。
Objective To investigate the genetic characteristics and molecular epidemiological characteristics of Echo30VP1 region of encephalitis isolates from 2008 in Kaifeng area. Methods Eight strains of Echo30 virus isolated from cerebrospinal fluid samples of viral encephalitis surveillance in Kaifeng area were sequenced and compared with the VP1 region of ECHO30 published on GeneBank. By constructing phylogenetic tree It carries out a genetic evolutionary analysis. Results The 8 Echo30VP1 genes were all 876bp in length and the translated amino acids were 292aa in length. The nucleotide and amino acid identities of VP1 of the 8 isolates were 92.8% -99.7% and 93.0% -99.7%, respectively. Genomic alignment with GenBank showed that all the isolates in 2008 in Kaifeng area were similar to those in Jiangsu province The homology of the isolates was high in the year. The evolutionary analysis indicated that it belonged to the seventh group. Conclusion In 2008, the Echo30 isolate in Kaifeng region was closest to the Jiangsu isolate in 2003 and may have the same origin.