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目的 :研究肺鳞癌、腺癌与Ki-ras基因外显子 1,2及p5 3基因外显子 7,8突变谱并观察肺鳞癌、腺癌预后同Ki-ras和 p5 3基因突变的关系。方法 :用PCR和测序方法分析 33例肺鳞癌和 2 7例肺腺癌Ki-ras基因外显子 1,2和 p5 3基因外显子 7,8错义突变。并追踪观察突变者和非突变者的二年存活率。结果 :肺鳞癌中发现 5例 (15 .16 % )Ki-ras基因突变和 12例 (36 .37% )p5 3基因突变 ;肺腺癌中发现 2例 (7.4 1% )Ki-ras基因突变和 7例 (2 5 .93% ) p5 3基因突变 ,两型肺癌间Ki-ras和p5 3基因突变阳性率差异均无统计学意义 (P>0 .0 5 ) ;肺鳞癌、腺癌中 p5 3基因突变总阳性率为 31.6 7% ,Ki-ras基因突变总阳性率仅 11.6 7% ,p5 3基因突变率高于Ki-ras基因突变率 (P <0 .0 5 )。p5 3基因突变谱与一般报道相似。Ki-ras基因突变情况与一般报道有所不同 ,未发现一般文献报道较多的第 12位等密码子突变 ,发现有第 6位密码子纯合性突变和第 31位和第 79位密码子杂合性突变。预后追踪显示 :无突变的病例 2年生存率为 4 0 % ,存在突变的病例 2年生存率仅 12 %。结论 :肺鳞癌、腺癌的Ki-ras和 p5 3基因突变谱较广 ,肺鳞癌和腺癌的预后均与这两个基因突变有关。
Objective: To study the mutation of exon 1, 2 and 5 of exon 1 and exon 7 of Ki-ras gene in lung squamous cell carcinoma, adenocarcinoma and Ki-ras gene and observe the prognosis of lung squamous cell carcinoma and adenocarcinoma with Ki-ras and p5 3 gene mutation Relationship. Methods: The missense mutations of exons 1, 2 and 5 of exon 1 and exon 7 of Ki-ras gene were detected by PCR and sequencing in 33 cases of lung squamous cell carcinoma and 27 cases of lung adenocarcinoma. Two-year survival rates of mutants and non-mutants were tracked. Results: Ki-ras gene mutation and 12 (36.37%) p5 3 gene mutations were found in squamous cell carcinoma of lung. Two cases (7.41%) of Ki-ras gene were found in lung adenocarcinoma There was no significant difference in the positive rate of Ki-ras and p5 3 mutations between the two types of lung cancer (P> 0.05), lung squamous cell carcinoma The positive rate of p5 3 gene mutation in cancer was 31.6 7%. The overall positive rate of Ki-ras gene mutation was only 11.6 7%. The mutation rate of p5 3 gene was higher than that of Ki-ras gene mutation (P <0.05). p5 3 gene mutation profile similar to the general report. Ki-ras gene mutation situation is different from the general report, found no more commonly reported in the literature such as the first 12 codon mutations found at codon 6 homozygous mutation and 31 and 79 codons Heterozygous mutation. The follow-up of the prognosis shows that the 2-year survival rate of the non-mutated cases is 40%, and the 2-year survival rate of the mutated cases is only 12%. Conclusions: The Ki-ras and p53 gene mutations in lung squamous cell carcinoma and adenocarcinoma are broad, and the prognosis of squamous cell carcinoma and adenocarcinoma are related to the mutation of these two genes.