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本文报道从血浆一步提取测定硝酸异山梨醇酯(ISDN)血药浓度的电子捕获-气相层析法,可供生物利用度和药物代谢动力学研究用的几千个样品的测定。1ml血浆与内标(5μl三硝酸甘油的乙酸乙酯溶液,1μg/ml)混和并与重蒸汽油(10ml)旋转振摇5分钟,离心分层,将上层汽油层完全转移至具塞试管中。于40°、氮气流下蒸发汽油至50μl。取2μl注入气相层析仪。电子捕获(~(63)Ni)气相层析仪为6尺×2.5mm(内径)新玻璃柱,用0F_1(3.5%)涂于Gas Chrom Q担
In this paper, an electron capture-gas chromatographic method for the determination of plasma concentrations of isosorbide dinitrate (ISDN) from one-step plasma was reported for the determination of several thousand samples for bioavailability and pharmacokinetic studies. 1 ml of plasma was mixed with an internal standard (5 μl of a solution of glyceryl trinitrate in ethyl acetate, 1 μg / ml) and shaken with heavy steam (10 ml) for 5 minutes, centrifuged and the upper petrol layer was completely transferred into a stoppered tube . Gas was evaporated to 40 [mu] l at 40 [deg.] Under nitrogen flow. Take 2μl into the gas chromatograph. The electron capture (~ 63 Ni) gas chromatograph was a 6-foot by 2.5-mm (id) new glass column coated with 0F_1 (3.5%) on Gas Chrom Q