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目的:研究窄叶芍药的化学成分,并对分离得到的化合物进行抗氧化活性评价。方法:采用反复硅胶柱色谱法和Sephadex LH-20柱色谱法等进行分离纯化,并通过理化常数测定和光谱分析鉴定其化学结构;并采用1,1-二苯基-2-硝基苯肼自由基清除法(DPPH)和2,2-联氮基-双-(3-乙基苯并噻唑啉磺酸-6)二铵盐自由基清除法(ABTS)对化合物的体外活性进行评价。结果:从窄叶芍药中分离得到5个化合物,并鉴定为没食子酸乙酯(1),没食子酸甲酯(2),齐墩果酸(3),丁香酸(4),β-谷甾醇(5);对化合物1~4进行抗氧化活性测试,结果表明:阳性药(维生素C,VC)及化合物1和2对DPPH自由基的半数清除率(IC50)分别达到(35.13±0.28),(44.64±0.37)μmol·L-1,均低于阳性对照抗坏血酸IC50(60.17±0.98)μmol·L-1;阳性药及化合物1和2对ABTS自由基的IC50分别达到(25.13±0.68),(35.76±1.48)μmol·L-1,均低于阳性对照抗坏血酸IC50(38.55±0.78)μmol·L-1。结论:化合物3,4首次从该植物中分离得到,并且化合物1和化合物2具有很强的抗氧化活性,其余化合物的抗氧化活性较弱。
OBJECTIVE: To study the chemical constituents of Paeonia lactiflora and evaluate the antioxidant activity of the isolated compounds. Methods: The compounds were isolated and purified by repeated silica gel column chromatography and Sephadex LH-20 column chromatography. Their chemical structures were identified by physicochemical constants and spectral analysis; and 1,1-diphenyl-2-nitrophenylhydrazine was used. DPPH and 2,2-diazoxy-bis-(3-ethylbenzothiazolinesulfonic acid-6) diammonium radical scavenging method (ABTS) were used to evaluate the in vitro activity of the compounds. RESULTS: Five compounds were isolated from the narrow-leaf peony drug and identified as ethyl gallate (1), methyl gallate (2), oleanolic acid (3), syringic acid (4), beta-sitosterol (5) Compounds 1-4 were tested for their antioxidant activity. The results showed that the half-clearance rates (IC50) of DPPH radicals of the positive drugs (vitamin C, VC) and compounds 1 and 2 reached (35.13±0.28), respectively. (44.64±0.37) μmol·L-1, lower than the IC50 of the positive control ascorbic acid (60.17±0.98) μmol·L-1; the IC50 of the positive drug and compound 1 and 2 to ABTS free radical reached (25.13±0.68), respectively. (35.76±1.48) μmol·L-1, lower than the IC50 of the positive control ascorbic acid (38.55±0.78) μmol·L-1. CONCLUSION: Compounds 3, 4 were isolated from this plant for the first time, and Compound 1 and Compound 2 had strong antioxidant activity, and the rest of the compounds had weak antioxidant activity.