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羊草是分布在我国北方的多年生优良禾本科牧草。但目前羊草退化严重。为了改良羊草的遗传性状,我们试图通过组织培养途径获得羊草新类型。作为这个工作的第一步,我们研究了由羊草根茎外植体诱导产生愈伤组织和再生植株的过程,现将结果简介如下。材料和方法诱导愈伤组织所用的外植体取自当年生羊草根茎幼嫩部分的节间基部切段以及隔年生老根茎和当年生根茎的节间中部、顶部切段。
Leymus chinensis is perennial fine gramineous pasture distributed in northern China. However, the current serious degeneration of Leymus chinensis. In order to improve the genetic traits of Leymus chinensis, we tried to obtain new types of Leymus chinensis by tissue culture. As a first step in this work, we investigated the process of callus induction and regeneration of plants induced by the rhizomatous explants of Leymus chinensis. The results are summarized below. MATERIALS AND METHODS The explants used in callus induction were obtained from the internode cuttings at the young part of the rhizome and the middle part of the internode and the top of the internode rhizome at the second year of life.