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研究了以全池泼洒的投药方式,孔雀石绿(MG)(池塘中MG的理论浓度为1 mg/L)及其主要代谢物隐性孔雀石绿(LMG)在斑点叉尾(Ietalurus punetaus)肌肉和皮肤以及养殖水体和底泥中的残留消除规律。采用高效液相色谱串联质谱法(HPLC-MS/MS)分析MG及其代谢物LMG在斑点叉尾体内及环境中的浓度水平。结果显示:肌肉、皮肤中MG于用药后第1天最高浓度分别为:(42.77±5.26)μg/kg和(6.36±0.11)μg/kg,消除半衰期T1/2分别为57.76 d、31.51 d;皮肤和肌肉中LMG分别在用药后第3天和第1天达到最高(502.27±20.43)μg/kg和(125.26±12.76)μg/kg,消除半衰期T1/2分别为33.01 d、38.51 d。这表明MG在斑点叉尾体内会迅速转化为LMG,且LMG残留在皮肤中的浓度大于肌肉中的浓度。养殖环境底泥中同时存在MG和LMG,以LMG为主,并且LMG呈现蓄积的趋势,在第360天出现最高浓度(5.92±1.23)μg/kg;水体中MG最高浓度出现在第1天,为(46.44±7.39)μg/L,随后急剧降至1μg/L左右,水体中几乎不存在LMG。
The effects of chuanchuan injection on the growth of Ietalurus punetaus (MG) (MG in ponds at a theoretical concentration of 1 mg / L) and its major metabolite, latent malachite green (LMG) Muscle and skin, as well as the rules of elimination of residuals in aquaculture water and sediments. The concentration of MG and its metabolite LMG in I. channel catfish and its environment were analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). The results showed that the maximal concentrations of MG in muscle and skin on day 1 were (42.77 ± 5.26) μg / kg and (6.36 ± 0.11) μg / kg respectively, and the elimination half-life T1 / 2 was 57.76 days and 31.51 days respectively. LMG in skin and muscle reached the highest (502.27 ± 20.43) μg / kg and (125.26 ± 12.76) μg / kg on day 3 and day 1, respectively. The elimination half-lives T1 / 2 were 33.01 d and 38.51 d, respectively. This indicates that MG rapidly converts to LMG in the channel catfish and that LMG remains in the skin at a greater concentration than in muscle. In the culture environment, there were both MG and LMG in the sediment, mainly LMG, and LMG showed the trend of accumulation, with the highest concentration (5.92 ± 1.23) μg / kg on the 360th day; the highest concentration of MG in water appeared on the first day, (46.44 ± 7.39) μg / L, then dropped sharply to 1 μg / L or so, with almost no LMG in water.