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This study examines the relationship among family supportive culture, organizational attachment, and work-life segmentation in high-tech service industry in Taiwan, China. Using survey data from 369 professionals, this study shows that family supportive culture has significant influence on organizational attachment, namely, affective commitment. Results indicate that individuals' work-life segmentation has a significant negative effect on organizational attachment. Meanwhile, our results further apply employees'segmentation between work and life as a moderator to investigating the impact of individual's perceptions of family supportive culture and values on a sense of attachment toward organizations. The result illustrates that work-life segmentation does not moderate the relationship between supportive family culture and employees' organizational attachment. Findings from this research provide insights into the influence of organizational family supportive culture and how it may further encourage employees' organizational attachment in high-tech industry in Taiwan.