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通过比较冬季不同海区锯缘青蟹(Scylla paramamosain)细胞膜脂肪酸组成,旨在理解冬季低温对甲壳动物细胞膜脂肪酸组成的影响。利用气相色谱法测定采自厦门和宁海海区锯缘青蟹鳃、肝胰腺和肌肉细胞膜中脂肪酸组成。结果显示:锯缘青蟹鳃和肝胰腺细胞膜中C16∶0和C16∶1在宁海海区显著低于厦门海区(P<0.01或P<0.05);肝胰腺细胞膜中C18∶0在宁海海区显著低于厦门海区(P<0.01);C20∶4在鳃、肝胰腺和肌肉细胞膜中宁海海区均显著高于厦门海区(P<0.01或P<0.05);肝胰腺中C20∶5宁海海区也显著高于厦门海区(P<0.01)。鳃和肝胰腺细胞膜中饱和指数∑SFA/∑UFA宁海海区显著低于厦门海区(P<0.01或P<0.05);肌肉细胞膜中无显著差异(P>0.05)。结果表明:冬季温度较低的宁海海区,细胞膜脂肪酸组成中不饱和脂肪酸增加,饱和指数下降,从而维持细胞膜的流动性和正常生理功能的执行。研究亮点:本研究率先以采自冬季不同海区的锯缘青蟹为研究对象,比较其鳃、肝胰腺和肌肉中细胞膜脂肪酸组成,研究冬季自然低温对青蟹细胞膜脂肪酸组成影响;探讨不同器官组织细胞膜在冬季低温适应上的差异,为青蟹北移驯养和越冬提供理论依据和生产指导。
The aim of this study is to understand the effects of winter cold on the fatty acid composition of the crustacean cell membrane by comparing the fatty acid composition of the membrane of Scylla paramamosain in different sea areas in winter. Determination of Fatty Acids in Gill, Hepatic Pancreas and Muscle Cell Membrane from Scylla serrata collected from Xiamen and Ninghai Sea Areas by Gas Chromatography. The results showed that C16:0 and C16:1 in the gill and hepatopancreas membrane of Scylla serrata were significantly lower in Ninghai sea area than in Xiamen sea area (P <0.01 or P <0.05), and C18:0 in hepatopancreas cell membrane was significantly lower in Ninghai sea area (P <0.01 or P <0.01). C20:4 was significantly higher in the gill, hepatopancreas and muscle cell membrane in Ninghai sea area than in Xiamen sea area (P <0.01 or P <0.05). C20:5 in the hepatopancreas was also significantly higher in Ninghai sea area In Xiamen sea area (P <0.01). The saturated index ΣSFA / ΣUFA in gill and hepatopancreas membrane was significantly lower in Ninghai sea area than in Xiamen sea area (P <0.01 or P <0.05), but no significant difference in muscle cell membrane (P> 0.05). The results showed that unsaturated fatty acids in the membrane fatty acid composition increased and saturation index decreased in the Ninghai Sea with lower temperature in winter so as to maintain the fluidity of the cell membrane and the normal physiological functions. Research highlights: This study took the first collected from different regions of the winter sea cynomolgus cynanchum as the research object, compare the gill, hepatopancreas and muscle cell membrane fatty acid composition, the study of natural low temperature in winter crab crab cell membrane fatty acid composition; explore different organs and tissues Cell membrane in the winter to adapt to the differences in low temperature for the crab crab northward domestication and winter provide a theoretical basis and production guidance.