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目的研究食物中毒沙门菌分离株的分子生物学特征,结合耐药表型,分析菌株间的同源性及耐药关系,为预防控制食源性疾病发生和临床治疗提供科学依据。方法对分离自2011—2016年石家庄市食物中毒食品或标本的40株沙门菌进行药敏检测,并采用脉冲场凝胶电泳(PFGE)进行分子分型,运用BioNumeries软件分析菌株之间的流行病学关系。结果 40株食源性沙门菌经Xba Ⅰ酶切可分为11种带型,药敏结果呈现多重耐药趋势。结论石家庄市食源性沙门菌呈高度散发、多重耐药的特点,特别是优势血清型肠炎沙门菌耐药性较明显,提示应加强沙门菌耐药性监测。
Objective To study the molecular biological characteristics of food poisoning Salmonella isolates, combine with drug resistance phenotype, analyze the homology and drug resistance among strains, and provide scientific basis for prevention and control of foodborne diseases and clinical treatment. Methods Forty Salmonella strains isolated from food poisoning samples in Shijiazhuang during 2011-2016 were tested for susceptibility and molecular typing by pulsed-field gel electrophoresis (PFGE). BioNumeries software was used to analyze the epidemiology of the strains Learning relationship. Results Forty Salmonella strains were digested with Xba Ⅰ and divided into 11 bands. The results of multidrug resistance showed drug susceptibility. Conclusion The food-borne Salmonella in Shijiazhuang City is characterized by highly dispersed and multi-drug resistance, especially the predominant serotype of Salmonella enteritidis, which suggests that Salmonella resistance should be strengthened.