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目的探讨不同厂家醒脑静注射液中挥发性主要成分的差异。方法采用手性气相色谱法分析,Astec CHIRALDEXTM B-DM(30 m×0.25 mm,0.12μm)毛细管手性气相色谱柱;进样口温度为250℃;FID检测器温度为280℃;炉温采用程序升温:起始温度60℃,10℃·min-1上升至130℃,再以2℃·min-1升至150℃,3℃·min-1升至210℃保持1 min。结果市场主流产品醒脑静注射液(无锡山禾和河南天地)成分存在较大差异,两者都含有麝香酮,但冰片成分差异很大,无锡山禾醒脑静中为左旋龙脑、左旋樟脑;河南天地则为右旋龙脑、右旋樟脑。结论该法分离效果良好,所鉴定成分准确,手性气相色谱法可为醒脑静注射液的深入研究提供依据。市售醒脑静不同厂家的成分不同,质量差异很大,应引起对中药手性成分作用的重视。
Objective To investigate the differences of volatile components in xingnaojing injection of different manufacturers. Methods Chiral gas chromatographic analysis was performed on a Chiral Gas Chromatography column with Astec CHIRALDEX ™ B-DM (30 m × 0.25 mm, 0.12 μm); inlet temperature was 250 ℃; FID detector temperature was 280 ℃; The temperature of the program was raised to 60 ° C, 10 ° C · min-1 to 130 ° C, then to 150 ° C at 2 ° C · min-1 and to 210 ° C at 3 ° C · min-1 for 1 min. Results There were significant differences in the components of Xingnaojing injection (Wuxi Shanhe and Henan Tiandao), both of which contained musk ketone. However, the composition of borneol was quite different. Camphor; Henan world is dextrorotatory, right-handed camphor. Conclusion The method has good separation effect, accurate identification of ingredients, and chiral gas chromatography can provide the basis for further study of Xingnaojing injection. Market Xingnaojing different manufacturers of different compositions, the quality is very different, should cause the role of traditional Chinese medicine, the importance of chiral ingredients.