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目的探讨地塞米松对胎粪吸入幼兔肺组织中肿瘤坏死因子-α(TNF-α)和白介素-8(IL-8)水平的影响。方法将24只日龄20～30天的幼兔随机分为对照组、生理盐水组和地塞米松组各8只,对照组气管插管后不灌胎粪;后两组气管插管后注入4 ml/kg的45 mg/ml胎粪混合物,地塞米松组在胎粪灌入后1 h和3 h静脉注射地塞米松0.5 mg/kg,生理盐水组在同样时间静脉注射等量的生理盐水;3组均在呼吸机比例辅助通气模式下通气,维持潮气量4～6 ml/kg、血氧饱和度85%以上和呼气末二氧化碳分压35～45 mm Hg为目标调节呼吸机参数,8 h后处死。取肺组织匀浆和支气管肺泡灌洗液(BALF),并用酶联免疫吸附试验测定TFN-α和IL-8的含量。结果对照组BALF和肺组织匀浆中TNF-α、IL-8含量均低于地塞米松组和生理盐水组,地塞米松组低于生理盐水组[BALF中TNF-α(pg/ml):(29.2±2.0)、(47.8±3.8)、(59.2±4.4),IL-8(pg/ml):(297.4±10.9)、(444.3±48.6)、(596.1±90.8);肺组织匀浆中TNF-α:(40.2±2.3)、(57.4±6.2)、(76.2±7.7),IL-8:(187.9±11.0)、(485.3±30.2)、(645.0±13.7)],差异有统计学意义(P均<0.05)。结论地塞米可以降低幼兔胎粪吸入肺组织匀浆和BALF中TNF-α和IL-8的水平,减轻肺损伤。
Objective To investigate the effect of dexamethasone on the levels of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) in the lungs of young rabbits. Methods Twenty-four young rabbits aged from 20 to 30 days were randomly divided into control group, saline group and dexamethasone group, and control group received intratracheal intratracheal intratracheal intratracheal instillation of feces. The latter two groups were injected intratracheally 4 ml / kg of 45 mg / ml meconium mixture. Dexamethasone group was injected intravenously with dexamethasone 0.5 mg / kg at 1 h and 3 h after meconium infusion. At the same time, saline group received the same amount of physiological Saline, and ventilated in proportion ventilation mode of ventilator to maintain the tidal volume 4 ~ 6 ml / kg, the oxygen saturation 85% and the end-tidal carbon dioxide partial pressure 35 ~ 45 mm Hg , 8 h after sacrifice. Pulmonary tissue homogenates and bronchoalveolar lavage fluid (BALF) were collected and the levels of TFN-α and IL-8 were measured by enzyme-linked immunosorbent assay. Results The levels of TNF-α and IL-8 in BALF and lung homogenate of control group were lower than that of dexamethasone group and saline group, while the dexamethasone group was lower than that of saline group [TNF-α (pg / ml) (29.2 ± 2.0), (47.8 ± 3.8), (59.2 ± 4.4), IL-8 (297.4 ± 10.9), (444.3 ± 48.6) and (596.1 ± 90.8) (40.2 ± 2.3), (57.4 ± 6.2), (76.2 ± 7.7), IL-8: (187.9 ± 11.0), (485.3 ± 30.2), (645.0 ± 13.7), respectively. The difference was statistically significant Significance (P <0.05). Conclusion Dexamethasone can reduce the levels of TNF-α and IL-8 in the aspirated lung tissue and BALF of young rabbits, and relieve lung injury.