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采用13对SSR引物,运用Bioptic Qsep100全自动核酸分析系统,分析了天目山5个海拔檫木群体的遗传多样性和遗传结构及其在不同海拔下的变化模式.结果表明:天目山檫木群体具有较高的遗传多样性水平,其中期望杂合度和观察杂合度分别为0.84和0.61.根据Shannon指数,天目山檫木中海拔(500~800 m)群体的遗传多样性水平大于低海拔(200 m)和高海拔(1100~1400 m)群体的遗传多样性水平.由基因分化系数和AMOVA分析可知,檫木种群的遗传变异主要存在于群体内.STRUCTURE分析和UPGMA聚类分析表明,中、低海拔被划为一个群体,而高海拔被划为另一个群体.低海拔和中海拔檫木群体遗传距离的差异表明,人为干扰对物种多样性具有负面效应,而自然保护区对物种多样性的保护起到了积极作用.
13 pairs of SSR primers were used to analyze the genetic diversity and genetic structure of Alnus cremastogyne population at 5 altitudes and the change patterns at different altitudes using Bioptic Qsep100 automated nucleic acid analysis system.The results showed that Alnus cremastogyne populations in Tianmu Mountain were High level of genetic diversity, the expected heterozygosity and observed heterozygosity were 0.84 and 0.61, respectively.According to Shannon’s index, the genetic diversity of Alnus cremastogyne in the range of 500 ~ 800 m was higher than that of the low altitude (200 m) The genetic diversity of high altitude (1100 ~ 1400 m) population was analyzed by genetic differentiation coefficient and AMOVA analysis.The genetic variation of Alnus creosote population mainly existed in the population.STRUCTURE analysis and UPGMA cluster analysis showed that the middle and low altitude Is divided into one group and high altitude is classified as another group.The difference of genetic distance between low altitude and middle altitude Alniphyllum showed that man-made interference has a negative effect on species diversity, and the protection of species diversity To a positive effect.