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本研究共收集95例罹患严重急性呼吸综合征(SARS)的医护人员的急性期和恢复期血清,用ELISA方法测定血清中白细胞介素2(IL-2)、白细胞介素4(IL-4)、白细胞介素10(IL-10)、干扰素γ(IFN-γ)、肿瘤坏死因子β(TNF-β)以及转化生长因子β(TGF-β)水平,用流式细胞仪测定急性期患者外周血CD4+和CD8+T细胞比例。结果显示,SARS患者病程初、中期血清IFN-γ无明显改变;IL-2在起病2月内无显著变化,但在病程3~5个月时降低;IL-4在健康对照和患者均未能测出;TNF-β在SARS感染的1周内和病程第2个月内下降。与上述细胞因子形成对比,患者IL-10以及TGF-β均在发病起即持续升高直至整个病程。这些细胞因子的变化均与激素的使用无关(P>0.05)。SARS患者急性期CD4+T细胞和CD8+T细胞急剧减少。可见,SARS相关冠状病毒感染可引起Th2细胞因子反应,可能损害患者的细胞免疫功能而促进体液免疫,IL-10和TGF-β可能在SARS的发病中起作用。
A total of 95 acute and convalescent sera from medical staff suffering from severe acute respiratory syndrome (SARS) were collected in this study. Serum levels of interleukin 2 (IL-2), interleukin 4 (IL-4) ), Interleukin 10 (IL-10), interferon gamma (IFN-gamma), tumor necrosis factor beta (TNF-beta) and transforming growth factor beta (TGF-beta) were detected by flow cytometry The proportion of CD4 + and CD8 + T cells in peripheral blood of patients. The results showed that there was no significant change of serum IFN-γ in early stage and intermediate stage of SARS patients; IL-2 had no significant change within 2 months of onset, but decreased in 3 ~ 5 months of course; IL-4 in both healthy controls and patients Failed to detect; TNF-β in SARS infection within 1 week and duration of the second month decreased. In contrast to the above cytokines, both IL-10 and TGF-β in patients are continuously elevated from onset to full course of disease. The changes of these cytokines have nothing to do with the use of hormones (P> 0.05). Acute phase of SARS patients with acute depletion of CD4 + T cells and CD8 + T cells. It can be seen that SARS-associated coronavirus infection can cause Th2 cytokine response, which may impair the cellular immune function of patients and promote humoral immunity. IL-10 and TGF-β may play a role in the pathogenesis of SARS.