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目的:研究不同炮制方法对四川朱砂莲中马兜铃酸A含量的影响。方法:采用4种不同的炮炙去毒方法对朱砂莲样品进行处理后,采用HPLC法对其马兜铃酸A的含量进行检测,并与生品中所含马兜铃酸A的含量进行分析比较。色谱条件:色谱柱为Agilent-C18柱(4.6mm×150mm,3.5μm),流动相:0.1%碳酸铵溶液-乙腈(72∶28),流速:1mL·min-1,检测波长250nm,柱温为28℃。结果:除蒸制组外,经蜜炙、甘草汁炙、碱水炙处理的四川朱砂莲样品与生品比较,马兜铃酸A的含量差异显著,有统计学意义(P<0.01),变化率超过80%。结论:蜜炙、甘草汁炙、碱水炙在四川朱砂莲的炮制处理中起到了很好的减毒作用。
Objective: To study the effect of different processing methods on the content of aristolochic acid A in Zusanli. Methods: Four different samples were used to treat the samples of Zhushaliu, then the content of aristolochic acid A was detected by HPLC and the contents of aristolochic acid A analyse and compare. The chromatographic conditions were as follows: Agilent-C18 column (4.6 mm × 150 mm, 3.5 μm), mobile phase of 0.1% ammonium carbonate and acetonitrile (72:28), flow rate of 1 mL · min-1 and detection wavelength of 250 nm. 28 ° C. Results: The content of aristolochic acid A was significantly different (P <0.01) compared with the raw samples of the samples treated with honeydew, licorice juice and alkaline water, The rate of change is over 80%. Conclusion: honey Sunburn, licorice juice Sunburn, Alkali Sunburn in the processing of the Chinese fir sand lotus played a very attenuated role.