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目的:探讨伏隔核微注射orexin-A后,大鼠摄食和活动的变化。方法:采用SD大鼠(250-280g),用脑立体定位仪在伏隔核植入微量注射管。大鼠随机分组,分别微注射乳酸格林液(Ringer’s),orexin-A 100pmol和500pmol。观察微注射后大鼠0-1h,1-2h,2-4h摄食和0-30min,30-60min,60-90min,90-120min活动性变化。结果:Orexin-A微注射后,大鼠0-1h,1-2h摄食量增加;30-60min,60-90min,90-120min的活动性显著增加(P<0.05 vs对照组)。结论:伏隔核是orexin-A刺激大鼠增加摄食量,提高其活动性的作用点。
Objective: To investigate the changes of ingestion and activity of rats after microinjection of orexin-A into nucleus accumbens. Methods: Sprague-Dawley rats (250-280g) were implanted with microinjectors in the nucleus accumbens by means of brain stereotactic apparatus. Rats were randomly assigned to Ringer’s, orexin-A 100 pmol and 500 pmol respectively. After the microinjection, the animals were ingested for 0-1h, 1-2h, 2-4h, and then for 30-30min, 60-60min, 90-120min. Results: Orexin-A microinjection increased the food intake of rats for 0-1h and 1-2h, and significantly increased the activity of 30-60min, 60-90min and 90-120min (P <0.05 vs. control group). Conclusion: The nucleus accumbens is the role of orexin-A stimulating rats to increase food intake and increase their activity.