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从安徽省某养殖场患病斑点叉尾鮰体内分离出6株细菌,经细菌形态、培养特性、生理生化特性测试,鉴定5株为爱德华氏菌,分别为AHIE01、AHIE02、AHIE03、AHIE04和AHIE05;1株为志贺氏菌,为AHIE06。经胸腔人工注射感染试验,证明所有分离菌株均具较强的致病性,可使斑点叉尾鮰100%发病;经再次进行病原分离鉴定,可从发病鮰鱼体内重新分离到生理生化特性与原分离菌株相同的细菌。用20种抗生素对分离菌株进行药物敏感性试验,结果表明,所有分离菌株均有不同程度的耐药性,并存在交叉耐药性和多重耐药性现象。
Six strains of bacteria were isolated from diseased Ictalurus punctatus at a farm in Anhui Province. Five strains of E. coli were identified as AHIE01, AHIE02, AHIE03, AHIE04 and AHIE05, respectively, after being tested for their morphological characteristics, culture characteristics and physiological and biochemical characteristics. Strain is Shigella, AHIE06. The intra-thoracic injection test showed that all isolates had strong pathogenicity and could cause 100% incidence of channel catfish. After re-pathogen isolation and identification, the physiological and biochemical characteristics and the original separation The same strain of bacteria. Twenty antibiotics were used to test the drug susceptibility of the isolates. The results showed that all isolates had different levels of drug resistance, and there were cross-resistance and multi-drug resistance.