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目的观察阳离子脂质体VEGF反义寡核苷酸对白血病细胞凋亡的作用。方法应用阳离子脂质体VEGFASODN转染HI60细胞48h后,观察细胞形态,电泳法检测DNA梯状带和流式细胞仪AnnexinVFITC/PI检测细胞凋亡。结果VEGFASODN组VEGFmRNA的表达完全抑制,VEGFMSODN组和空白对照组的VEGFmRNA的表达没有明显变化,细胞生长受抑制可见细胞固缩,DNA电泳出现典型的梯状条形带,流式细胞仪分析细胞凋亡率可达23.28%,明显高于VEGFMSODN组的8.21%和空白对照组。结论VEGFASODN可抑制白血病细胞VEGFmRNA的表达,导致白血病细胞的凋亡,VEGF基因与白血病细胞的凋亡有关。
Objective To observe the effect of cationic liposome VEGF antisense oligonucleotide on apoptosis of leukemia cells. Methods HI60 cells were transfected with liposomal VEGFASODN for 48 hours. The morphology of the cells was observed. DNA ladder and flow cytometry AnnexinVFITC / PI were used to detect the apoptosis of HI60 cells. Results The expression of VEGFmRNA in VEGFASODN group was completely inhibited. The expression of VEGFmRNA in VEGFMSODN group and blank control group did not change significantly. The cell growth was inhibited and the typical ladder stripe was observed by DNA electrophoresis. Flow cytometry The mortality rate was 23.28%, which was significantly higher than that in the VEGFMSODN group (8.21%) and the blank control group. Conclusion VEGFASODN can inhibit the expression of VEGFmRNA in leukemia cells and induce the apoptosis of leukemic cells. VEGF gene is related to the apoptosis of leukemic cells.