论文部分内容阅读
目的:探讨白细胞介素-1β(IL-1β)对鼻黏膜上皮细胞黏蛋白MUC2和MUC5BmRNA表达的影响。方法:在培养的第二代人鼻黏膜上皮细胞加入IL-1β(10μg/L)刺激24h后,采用荧光定量RT-PCR检测人鼻黏膜上皮细胞中MUC2和MUC5BmRNA的定量表达。结果:在培养的鼻黏膜上皮细胞上均检测到MUC2和MUC5BmRNA的表达,IL-1β刺激组MUC2mRNA定量表达[(39.26±6.10)×104拷贝/μg]高于对照组[(5.70±4.16)×104拷贝/μg],差异有统计学意义(P<0.01);IL-1β刺激组MUC5BmRNA定量表达[(5.72±2.06)×105拷贝/μg]高于对照组[(1.11±0.72)×105拷贝/μg],差异有统计学意义(P<0.05)。结论:在培养的鼻黏膜上皮细胞中IL-1β组的MUC2和MUC5BmRNA表达均高于对照组,提示IL-1β可能具有上调鼻黏膜上皮细胞黏蛋白mRNA的表达作用。
Objective: To investigate the effect of interleukin-1β (IL-1β) on the expression of mucins MUC2 and MUC5B mRNA in nasal mucosa epithelial cells. Methods: The expression of MUC2 and MUC5B mRNA in human nasal mucosa epithelial cells were detected by real-time fluorescence quantitative RT-PCR after the cultured human second generation human nasal epithelial cells were stimulated with IL-1β (10μg / L) for 24h. Results: The expression of MUC2 and MUC5B mRNA were detected in cultured nasal epithelial cells. The expression of MUC2 mRNA in the group of IL-1β stimulation [(39.26 ± 6.10) × 104 copies / μg] was significantly higher than that in the control group [(5.70 ± 4.16) × 104 copies / μg], the difference was statistically significant (P <0.01). The quantitative expression of MUC5B mRNA in IL-1β stimulated group [(5.72 ± 2.06) × 105 copies / μg] was higher than that in the control group [(1.11 ± 0.72) × 105 copies / μg], the difference was statistically significant (P <0.05). CONCLUSION: The expression of MUC2 and MUC5B mRNA in IL-1β group is higher than that of the control group in nasal mucosa epithelial cells, suggesting that IL-1β may up-regulate the expression of mucin mRNA in nasal mucosa epithelial cells.