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目的:观察以1%乙二醇+2%氯化铵配成的造石液制作大鼠肾草酸钙结石病模的表现,同时试验迷迭香酸对此病模的影响,为今后研究抗肾草酸钙结石的药物提供可靠的模型。方法:以1%乙二醇+2%氯化铵配成的造石液每天灌胃,连续28天,诱导大鼠草酸钙晶体形成,建立大鼠肾草酸钙结石模型,采用全自动生化分析仪测定大鼠尿生化、血生化,解剖取双肾,测定肾组织草酸、枸橼酸等指标。结果:模型组的尿生化、血生化、肾组织草酸均显著高于空白对照组、迷迭香酸组(P<0.01)。结论:以1%乙二醇+2%氯化铵成功建立大鼠肾草酸钙结石模型,迷迭香酸对结石病模有非常明显改善作用。应用此模型,对受试药物进行实验研究。
OBJECTIVE: To observe the manifestation of rat model of renal oxalate stones with 1% ethylene glycol + 2% ammonium chloride, and to study the effect of rosmarinic acid on this model. Calcium oxalate stones provide a reliable model of the drug. METHODS: The stone-forming fluid formulated with 1% ethylene glycol + 2% ammonium chloride was intragastrically administered for 28 consecutive days to induce the formation of calcium oxalate crystals in rat. The model of rat kidney oxalate calculus was established. The model was established by automatic biochemical analysis Instrument determination of urine biochemical, blood biochemical, anatomy and kidneys, determination of oxalic acid, citrate and other indicators of kidney tissue. Results: Urine biochemistry, blood biochemistry and oxalic acid in renal tissue in model group were significantly higher than those in blank control group and rosmarinic acid group (P <0.01). Conclusion: Rat kidney oxalate stone model was successfully established with 1% ethylene glycol + 2% ammonium chloride. Rosmarinic acid had a very significant improvement on the stone mold. Apply this model to the experimental drugs for experimental study.