目的：为了解＋ ＧＺ 重复暴露后大鼠脑组织ｃｆｏｓ、ｃｊｕｎ 和神经生长因子（ＮＧＦ） 基因表达的变化，探讨它们在＋ ＧＺ 所致脑损伤中的作用和意义。方法：用半定量反转录聚合酶链反应（ＲＴＰＣＲ） 检测方法检测＋ ＧＺ 重复暴露后大鼠脑组织ｃｆｏｓ、ｃｊｕｎ 和ＮＧＦ ｍ ＲＮＡ 表达水平。结果：ｃｆｏｓ 和ｃｊｕｎ 在＋ ＧＺ 重复暴露后３０ ｍｉｎ ｍ ＲＮＡ 表达明显升高，６ｈ 和２４ｈ 降至正常；ＮＧＦ 在３０ ｍｉｎ 和６ ｈ 表达均明显升高，２４ ｈ 恢复正常。结论：表明＋ ＧＺ 重复暴露可诱导大鼠脑组织ｃｆｏｓ、ｃｊｕｎ 和ＮＧＦ ｍ ＲＮＡ 的表达，提示它们的表达增加可能在＋ ＧＺ 所致脑损伤中起病理或保护作用。 Objective: To investigate the changes of gene expression of cfos, cjun and nerve growth factor (NGF) in rat brain after + GZ repeated exposure, and to explore their roles and significance in + GZ induced brain injury. Methods: The expression of c-fos, c-jun and NGF m RNA in rat brain after repeated G + exposure was detected by semi-quantitative reverse transcription-polymerase chain reaction (RTPCR). Results: The mRNA expression of cfos and cjun at 30 min after + GZ repeated exposure was significantly higher than that at 6 h and 24 h, while the expression of NGF at 30 min and 6 h was significantly increased and returned to normal at 24 h. CONCLUSION: Repeated exposure of + GZ can induce the expression of c-fos, c-jun and NGF m RNA in rat brain, indicating that their increased expression may play a pathological or protective role in + GZ-induced brain injury.