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目的:探讨转化生长因子1(TGFβ1)和上皮钙黏附素(Ecd)在声门上型喉鳞状细胞癌(SGLSCC)中的表达与颈部淋巴结转移和预后的关系。方法:采用免疫组织化学SABC法对60例SGLSCC蜡块标本和10例正常喉黏膜标本中TGFβ1、Ecd的表达情况进行检测,结合临床及随访资料进行分析。结果:①60例SGLSCC中TGFβ1和Ecd的阳性表达率分别为81.7%(49/60)和70.0%(42/60);10例正常喉黏膜中TGFβ1和Ecd的阳性表达率分别为20.0%(2/10)和90.0%(9/10);②TGFβ1和Ecd在淋巴结、病理分级及临床分期3项在各组间的表达分别呈正、负相关关系;③两指标的表达与淋巴结转移有显著的关系,而与患者的年龄、性别及肿瘤大小无关;④两指标间无一致性但呈负相关关系;⑤Ecd的表达与生存率曲线有统计学意义。结论:SGLSCC中TGFβ1和Ecd的表达与淋巴结转移有关,两指标间呈负相关关系,两指标可作为预测SGLSCC淋巴结转移的标志物,而Ecd还可作为判断预后的标志物。
Objective: To investigate the relationship between expression of TGFβ1 and Ecd in human supraglottic laryngeal squamous cell carcinoma (SGLSCC) and lymph node metastasis and prognosis. Methods: Immunohistochemical SABC method was used to detect the expression of TGFβ1 and Ecd in 60 specimens of SGLSCC and 10 specimens of normal laryngeal mucosa, and the clinical and follow-up data were analyzed. RESULTS: ① The positive rates of TGFβ1 and Ecd in 60 cases of SGLSCC were 81.7% (49/60) and 70.0% (42/60), respectively. The positive rates of TGFβ1 and Ecd in 10 cases of normal laryngeal mucosa were 20.0% (2) / 10) and 90.0% (9/10), respectively. (2) The expression of TGFβ1 and Ecd in the lymph nodes, pathological grading and clinical stage were positively and negatively related to each other. The expression of both indexes was significantly correlated with lymph node metastasis , But not with the patient’s age, gender and tumor size; ④ There is no consistency between the two indicators but negative correlation; ⑤Ecd expression and survival curve was statistically significant. CONCLUSIONS: The expressions of TGFβ1 and Ecd in SGLSCC are correlated with lymph node metastasis. There is a negative correlation between the two indexes, which can be used as a marker of lymph node metastasis in SGLSCC. Ecd can also be used as a marker of prognosis.