研究了切除雌性大鼠68％肝脏以后再生肝枯否细胞(KC)产生α肿瘤坏死因子(αTNF)的能力,并以单纯剖腹组为对照。手术均在上午8～10时进行,术后24小时以胶原酶消化大鼠肝脏,粘附法分离纯化KC。培养24小时,取含5×10~5KC的培养液1ml,与细菌脂多糖(LPS)一起培养。分别于8、12、24、48小时取上清液,以L929成纤维细胞溶解法测定αTNF活性。结果显示,假手术组5×10~5KC在用2.5μg/mlLPS刺激后8小时,αTNF产量明显 To investigate the ability of regenerating hepatic Kupffer cells (KC) to produce α-TNF (TNF-α) after excision of 68% of the liver in female rats. The control group was treated with Caesarean section alone. The surgery was performed at 8-10 am, and the rat liver was digested with collagenase 24 hours after operation. KC was isolated and purified by adhesion method. After culturing for 24 hours, 1 ml culture solution containing 5 × 10 -5 KC was cultured together with bacterial lipopolysaccharide (LPS). Supernatants were harvested at 8, 12, 24 and 48 hours, respectively, and αTNF activity was measured by L929 fibroblast lysis. The results showed that the sham operation group 5 × 10 ~ 5KC 2.5μg / ml LPS stimulation 8 hours, αTNF production was significantly