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应用多聚酶键反应—聚丙烯酰胺凝胶电泳（ＰＣＲ－ＰＡＧＥ）和银染色法对１６４例恶性血液病患者进行了免疫球蛋白重链基因（ＩｇＨ）、Ｔ细胞受体γ链基因（ＴＣＲγ）的克隆性重排检测。结果显示，重排之ＩｇＨ与ＴＣＲγ总比例为３∶１，ＡＬＬ进展期ＩｇＨ占５０％，ＴＣＲγ占２７．３％，双阳性１８．２％，总计阳性率为９５．５％。当骨髓象缓解时克隆重排仍占６０％，ＭＭ有８５％为ＩｇＨ重排，５％为ＴＣＲγ，缓解时仍有２５％呈ＩｇＨ重排。ＴＣＲγ及双阳性所占比例上升与病情恶化呈正相关。因此以这种检测方法来指导化疗可以提高生存率和治愈率。
Polyclonal enzyme reaction-polyacrylamide gel electrophoresis (PCR-PAGE) and silver staining were performed on 164 patients with hematological malignancies who had immunoglobulin heavy chain gene (IgH) and T cell receptor gamma chain gene (TCRγ) Clonal rearrangement detection. The results showed that the total proportion of IgH and TCRγ in the rearrangement was 3:1, IgH in advanced ALL stage was 50%, TCRγ was 27.3%, double positive was 18.2%, and the total positive rate was 95.5%. When the bone marrow was relieved, the clone rearrangement still accounted for 60%, 85% of the MM were IgH rearrangements, 5% were TCRγ, and 25% showed IgH rearrangement when remission. The increase in the proportion of TCRγ and double positive was positively correlated with the worsening of the disease. Therefore, the use of this test to guide chemotherapy can improve survival and cure rates.