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目的:测定不同产地桃儿七中鬼臼毒素和总木脂素类成分含量,对其资源利用价值进行评价。方法:采用高效液相色谱法和紫外分光光度法分别测定不同产地共126个桃儿七样品中鬼臼毒素和总木脂素含量,采用参比波长法测定桃儿七中总木脂素类成分的含量。结果:桃儿七中鬼臼毒素和总木质素的含量均以产自甘肃省永登县自然保护区样地的样品含量最高,产自西藏自治区江达同普乡样地的样品含量最低,前者分别为7.40%,20.66%,后者分别为0.40%,5.46%,两者分别相差近19倍和4倍。桃儿七中鬼臼毒素与总木脂素的含量呈显著正相关,且两者的含量表现出海拔较低、纬度较高地区样地的含量较高的特点。结论:2种含量测定方法均简便、快速、准确、重复性好;以鬼臼毒素含量和总木脂素含量为指标评价桃儿七资源利用价值较为客观;本研究的结果,对于桃儿七野生资源采集和栽培生产基地建设的产地选择具有重要的指导意义。
Objective: To determine the contents of podophyllotoxin and total lignans in different regions of Taoyuan seven, and to evaluate their resource utilization value. Methods: The content of podophyllotoxin and total lignin in 126 samples of peach seven from different habitats were determined by HPLC and UV spectrophotometry. The total lignans Content of ingredients. Results: The content of podophyllotoxin and total lignin in Taohong seven was the highest among the samples from the nature reserve of Yongdeng Nature Reserve in Gansu Province. The content of podophyllotoxin and total lignin in Taohe seven was the lowest, The former were 7.40% and 20.66% respectively, while the latter were 0.40% and 5.46% respectively, a difference of nearly 19 times and 4 times respectively. Peach seven in podophyllotoxin and total lignans content was significantly positive correlation, and both content showed the lower elevation, higher latitude area plots higher content characteristics. Conclusion: The method of determination of two kinds of content is simple, rapid, accurate and reproducible. Using podophyllotoxin content and total lignan content as index to evaluate the utilization value of Taoyu seven resources is more objective. The results of this study are as follows: The choice of the place of origin for the collection of wild resources and the cultivation and production base has an important guiding significance.