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目的检测酰基精氨酸脱亚氨酶4(PADI4)在类风湿关节炎(RA)患者外周血单个核细胞(PBMC)mRNA的表达,分析其与RA临床相关指标的相关性,探讨PADI4在RA发病机制中的作用。方法采用反转录聚合酶链反应(RT-PCR)实时荧光定量法对RA组(53例)、骨关节炎(OA)组(27例)及正常对照组(30名)PBMC中PADI4 mRNA表达进行检测,并分析其与抗环瓜氨酸(CCP)抗体、疾病活动关节评分28(DAS 28)、患者病程的关系。结果RA组PADI4 mRNA表达明显高于OA组和正常对照组,差异有统计学意义(P<0.05);PADI4 mRNA的表达在OA组、正常对照组差异无统计学意义(P>0.05)。RA组中PADI4 mRNA表达与抗CCP抗体和DAS28评分水平呈明显正相关(r=0.452,0.653,P均<0.05),与病程呈负相关(r=-0.68,P<0.05)。结论RA组PADI4 mRNA表达显著增高,并与DAS28评分和抗CCP抗体水平呈明显正相关,与病程呈负相关。提示RA患者PADI4 mRNA表达明显增高,与疾病活动程度和自身免疫功能异常相关。
Objective To detect the expression of PADI4 mRNA in peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA) and analyze its correlation with clinically relevant indicators of RA and to explore the role of PADI4 in RA The role of pathogenesis. Methods The expression of PADI4 mRNA in PBMC of RA group (53 cases), osteoarthritis (OA) group (27 cases) and normal control group (30 cases) were detected by reverse transcription polymerase chain reaction (RT- , And analyzed their association with anti-cyclic citrulline (CCP) antibody, disease activity joint score 28 (DAS 28), and patient duration. Results The expression of PADI4 mRNA in RA group was significantly higher than that in OA group and normal control group (P <0.05). There was no significant difference in PADI4 mRNA expression between OA group and normal control group (P> 0.05). The expression of PADI4 mRNA in RA group was positively correlated with anti-CCP antibody and DAS28 score (r = 0.452, 0.653, P <0.05), and negatively correlated with the course of disease (r = -0.68, P <0.05). Conclusions The expression of PADI4 mRNA in RA group is significantly increased, and positively correlated with DAS28 score and anti-CCP antibody level, but negatively correlated with the course of disease. These results suggest that the expression of PADI4 mRNA in RA patients is significantly increased, which is related to the degree of disease activity and the abnormality of autoimmune function.