论文部分内容阅读
本文用双参数FCM技术,对同一个细胞的DNA和RNA含量进行相关测量,比较了ACM B对小鼠L_(1210)白血病细胞周期和RNA含量的影响.结果发现在一次给药后8小时可导致早、中期S的积累,并抑制S期细胞的DNA合成;到24小时DNA合成恢复正常,并进入G_2期,但由于G_2期细胞进入M期受阻,造成G_2期细胞的积累,这时被阻断在G_2期的细胞RNA含量显著增加,形成正不平衡生长,而给药剂量较大的实验组(1/1.5LD_(50))S期细胞的RNA含量不随着DNA含量的增加而增加,形成负不平衡生长,ACM A和ACM B对体内Li_(210)细胞周期作用相同.
In this paper, we measured the DNA and RNA content of the same cell by two-parameter FCM technique and compared the effect of ACM B on the cell cycle and RNA content of L 1210 leukemia in mice.It was found that 8 hours after one administration Leading to the accumulation of early and mid-term S and inhibiting DNA synthesis in S phase cells. DNA synthesis returned to normal in 24 hours and entered the G2 phase. However, cells in G 2 phase were blocked during M phase and resulted in the accumulation of G 2 phase cells Blocking the G 2 phase cell RNA content increased significantly to form a positive unbalanced growth, while the dose of the larger experimental group (1 / 1.5LD_ (50) S phase cells RNA content does not increase with increasing DNA content , Resulting in negative imbalance growth. ACM A and ACM B have the same effect on Li 210 cell cycle in vivo.