论文部分内容阅读
为了解恩诺沙星在环境中残留对土壤微生物的影响,应用扩增核糖体DNA限制性分析(ARDRA)对土壤细菌16SrDNA基因多样性进行了研究,并结合肠杆菌基因间的重复共有序列(ERIC-PCR)指纹图谱分析了恩诺沙星对土壤细菌种群基因多样性的影响.结果表明:恩诺沙星作用于土壤后第35天,添加药物组的细菌总数均低于对照,且药物浓度越高,细菌数量越少;ARDRA分析将分离的土壤细菌分成了不同的操作分类单元(OTU),各组的OTUs类型数分别为:Ⅰ组15个、Ⅱ组13个、Ⅲ组10个、Ⅳ组8个、Ⅴ组6个、Ⅵ组6个;对各组优势OTU进行了ERIC-PCR基因指纹图谱分析,Ⅰ～Ⅵ组的Shannon-Wiener指数分别为2.78、2.14、1.78、1.11、0.69和0.31,对照组的Margalef指数、Simpson指数和Pielou指数均明显高于添加药物组,且各多样性指数随药物浓度的增加而减少.
In order to understand the effect of enrofloxacin on soil microorganisms in the environment, the 16S rDNA gene diversity of soil bacteria was studied by using amplified ribosomal DNA restriction analysis (ARDRA), and combined with the repeat consensus sequence of the Enterobacteriaceae ERIC-PCR) fingerprints were used to analyze the effect of enrofloxacin on the genetic diversity of soil bacterial populations.The results showed that on the 35th day after enrofloxacin was applied to the soil, the total number of bacteria in the drug group was lower than that of the control group, and the drug The higher the concentration, the smaller the number of bacteria. The ARDRA analysis divided the isolated soil bacteria into different operational taxonomic units (OTUs). The number of OTUs in each group was 15 in group I, 13 in group II and 10 in group III , 8 in group Ⅳ, 6 in group V and 6 in group VI. The fingerprinting of ERIC gene was performed on the dominant OTU in each group. The Shannon-Wiener indices of Ⅰ ~ Ⅵ group were 2.78, 2.14, 1.78, 1.11, 0.69 and 0.31, Margalef index, Simpson index and Pielou index of the control group were significantly higher than the additive group, and the diversity index decreased with the increase of the drug concentration.