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应用原位分子杂交及免疫组化方法,使用地高辛标记HCV5′非编码区探针及抗HCVNS3区C33c单克隆抗体,对35例人原发性肝内胆管细胞癌(PIC)和癌旁肝组织内HCVRNA及其NS抗原进行检测,结果发现HCVRNA在PIC中的阳性率为83%,HCVRNA定位于癌细胞胞浆中。个别病例并在淋巴细胞、肝窦内皮细胞和枯否氏细胞中发现阳性信号。HCVNS3区C33c抗原在PIC的阳性率为89%,阳性反应主要位于癌细胞胞浆内或肝细胞胞浆内。阳性细胞在癌组织中以灶性分布为主,在癌旁肝以弥漫分布为主。HBxAg在本组病例的阳性率为77%。结果提示:在肝内胆管细胞癌的发生中,除HBV以外.HCV感染与其发生也有密切关系,是其致病因素之一。
In situ hybridization and immunohistochemistry using digoxigenin-labeled HCV5′ non-coding region probe and anti-HCV NS3 region C33c monoclonal antibody against 35 cases of human primary intrahepatic cholangiocarcinoma (PIC) and adjacent cancer Detection of HCV RNA and NS antigens in liver tissue revealed that the positive rate of HCV RNA in PIC was 83%, and HCV RNA was localized in the cytoplasm of cancer cells. In individual cases, positive signals were found in lymphocytes, sinusoidal endothelial cells, and Kupffer cells. The positive rate of HCV NS3 C33c antigen in PIC was 89%, and the positive reaction was mainly located in the cytoplasm of cancer cells or cytoplasm of hepatocytes. The positive cells were mainly focally distributed in cancer tissues and diffusely distributed in adjacent livers. The positive rate of HBxAg in this group was 77%. The results suggest that: In the occurrence of intrahepatic cholangiocarcinoma, in addition to HBV. HCV infection is also closely related to its occurrence and is one of its pathogenic factors.