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采用人工投喂的方法对人工选育的104个“黄海2号”中国对虾(Fenneropenaeus chinensis)家系的5557尾个体[体重(2.1±0.7)g]进行WSSV感染实验,分别选取存活时间最长(342h)和存活时间最短(45h)的48尾个体,定义为抗病组和敏感组。用荧光定量PCR方法检测两组对虾体内WSSV含量,结果表明,抗病组和敏感组对虾体内WSSV含量范围分别为(2.64×104)—(3.13×106)和23.2—1.92×106copies/ngDNA,平均值分别为8.71×105和3.19×105copies/ng DNA,抗病组对虾WSSV含量显著高于敏感组(P<0.05),显示抗病组比敏感组具有更强的抗病性能。对感染测试的所有对虾的死亡情况进行统计分析,结果表明,其死亡曲线不符合数量性状的正态分布特征,提示中国对虾对WSSV的抗性可能由少数几个主基因决定。本研究可为揭示中国对虾抗WSSV性状的基因决定机制提供依据。
Five hundred and fifty-five57 individuals [body weight (2.1 ± 0.7) g] from 104 artificially-bred Fenneropenaeus chinensis families were tested for WSSV infection by artificial feeding. The survival time The 48 individuals with long (342h) and shortest survival (45h) were defined as resistant and susceptible. The results of real-time quantitative PCR showed that the WSSV contents of shrimp were (2.64 × 104) - (3.13 × 106) and 23.2-1.92 × 106copies / ngDNA, respectively, in the resistant and susceptible groups (8.71 × 105 and 3.19 × 105 copies / ng DNA, respectively). The WSSV content in shrimp was significantly higher than that in the susceptible group (P <0.05), which indicated that the disease resistance group had stronger disease resistance than the susceptible group. The statistical analysis of the deaths of all the shrimp infected by the infection showed that the death curve did not fit the normal distribution of quantitative traits, suggesting that the resistance of WSSV may be determined by a few major genes. This study may provide the basis for revealing the gene-determining mechanism of WSSV traits in Chinese shrimp.