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白细胞功能性成熟度的客观指标之一是细胞的密度,用等密度离心法,白细胞正常密度为1.095～1.120g/cm~3。外周血幼稚的粒细胞比成熟的白细胞密度低。作者用变应原孵育白细胞,用离心法测定粒细胞移动指数,评定机体过敏;因为变应原促使粒细胞脱粒导致密度降低。方法:取静脉血加抗凝剂(肝素10u/ml),每200～500μl 一份,份数取决于变应原数;实验组加变应原,对照组加缓冲生理盐水,试样在37℃孵育60分钟,加入
One of the objective indicators of the functional maturity of leukocytes is the density of the cells. The density of normal white blood cells is 1.095-1.120 g / cm ~ 3 by iso-density centrifugation. Immature naive granulocytes have a lower density of mature white blood cells. The authors used leukocytes to incubate with allergens, measured by centrifugation granulocyte migration index, assess the body allergy; because allergen prompting granulocyte threshing lead to reduced density. Methods: Venous blood plus anticoagulant (heparin 10u / ml), every 200 ~ 500μl, the number depends on the number of allergens; experimental group plus allergen, the control group with buffered saline, the sample in 37 Incubate for 60 minutes, add