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辣椒炭疽病菌(辣椒刺盘孢Colletotrichum capsici)在25—28℃的Czapek-Dox培养液中振荡培养时分泌出毒素,这种毒素能引起辣椒叶片形成坏死斑,类似于病原菌侵染形成的症状。辣椒叶煎汁培养液和Czapek-Dox培养液适于C.capsici的生长和产毒,生长适宜温度和范围分别为25℃和pH6—7,在25—28℃,pH6—7的条件下培养滤液毒性最强;光线和通气条件可促进C.capsici的生长和产毒;培养14天的培养滤液毒性最强。培养滤液经丙酮沉淀及离子交换树脂柱和Sephadex G-50柱层析将毒素纯化。实验结果表明该毒素为多聚糖类物质。生物检测结果表明:培养滤液和C.capsici毒素溶液能抑制辣椒、绿豆、豌豆、豇豆种子胚根生长;并能使辣椒幼苗发生萎蔫,这一作用与辣椒品种抗性有关。
Pepper anthracnose (Colletotrichum capsici) secreted toxins during shaking culture in Czapek-Dox medium at 25-28 ° C. This toxin causes the necrotic spots of pepper leaves to form, similar to the symptoms of pathogen infection. Capsicum frutescens broth and Czapek-Dox broth are suitable for the growth and toxigenicity of C. capsici at the optimum temperature and range of 25 ° C and pH 6-7, respectively, and cultured at 25-28 ° C, pH 6-7 The filtrate has the strongest toxicity. Light and ventilation conditions can promote the growth and toxin production of C. capsici. The culture filtrate cultured for 14 days has the strongest toxicity. The filtrate was purified from the filtrate by acetone precipitation and ion exchange resin column chromatography on a Sephadex G-50 column. The experimental results show that the toxins are polysaccharides. The results of biological tests showed that the culture filtrate and C. capsici toxin solution could inhibit the radicle growth of pepper, mung bean, pea and cowpea seeds and wilt the pepper seedlings, which was related to the resistance of pepper varieties.