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设计了针对ＨＢＶ（ａｄｒ亚型）ＲＮＡ的二个锤头状核酶（ＲＳ３和ＲＣ２），并将其插入ｔＲＮＡ反密码环中（ＲｔＳ３和ＲｔＣ２），以增加其稳定性。实验表明，虽然插入ｔＲＮＡ中的核酶与裸露核酶相比，催化活性有所下降，但在胎牛血清和ＨｅｐＧ２细胞抽提液中的稳定性却明显提高。因此，ｔＲＮＡ－包埋核酶有可能提高在体内的抗病毒能力。
Two hammerhead ribozymes (RS3 and RC2) directed against HBV (adr subtype) RNA were designed and inserted into tRNA anticodon loops (RtS3 and RtC2) to increase their stability. Experiments show that, although the insertion of tRNA ribozyme compared with the naked ribozyme catalytic activity decreased, but in fetal bovine serum and HepG2 cell extract stability was significantly improved. Therefore, tRNA-embedded ribozymes have the potential to increase antiviral capacity in vivo.