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水体盐度是影响鱼类生长的重要环境因素之一,为了解急性盐度胁迫对点篮子鱼(Siganus guttatus)存活率及肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性的影响,设定盐度30(对照)、盐度20、盐度10、盐度5和淡水5个盐度梯度进行试验.结果表明:96 h内各组死亡率均为0%,182 h淡水组死亡率为100%,其余4组死亡率为0%.酶活性检测结果显示:将点篮子鱼直接放入盐度10和盐度20的水体中,SOD、CAT和GSH-PX活性虽有一定程度的升高,但与对照组无显著性差异(P>0.05).直接放入盐度5的水体中,SOD酶活性与对照组无显著性差异,而CAT和GSH-PX活性与对照组有显著性差异,但在24 h时均恢复到对照组水平.直接放入淡水中,3种酶活性均在3 h时达到最高,与对照组有显著性差异,SOD活性在12 h时恢复到对照组水平,CAT活性在48 h时恢复到对照组水平,GSH-PX活性在48 h时与对照组仍有显著性差异(P<0.05),96 h时仍高于对照组,但无显著性差异(P>0.05).结果表明:点篮子鱼对低盐的耐受力比较强,在盐度突然改变时能通过调节自身的抗氧化酶活力来清除体内多余的自由基,但对淡水环境耐受力比较差.
The salinity of water body is one of the important environmental factors that affect the growth of fish. In order to understand the effects of acute salinity stress on the survival rate of Siguanus guttatus and the activities of hepatic superoxide dismutase (SOD), catalase (CAT) Glutathione peroxidase (GSH-PX) activity, the salinity 30 (control), salinity 20, salinity 10, salinity 5 and freshwater five salinity gradient were tested.The results showed that: The mortality rate in each group was 0% in 96 h, 100% in 182 h fresh water group, and 0% in the other 4 groups. The results of enzyme activity test showed that the basal fish were directly put into salinity 10 and salinity 20 in water body, SOD, CAT and GSH-PX activity had a certain degree of increase, but there was no significant difference with the control group (P> 0.05) .Directly into the salinity 5 of water, SOD activity and control Group and no significant difference in CAT and GSH-PX activities, but the activity of CAT and GSH-PX in control group were all significantly different at 24 h, but returned to the level of control group at 24 h. , And there was a significant difference with the control group. The SOD activity returned to the control level at 12 h, CAT activity returned to the control level at 48 h, GSH-PX activity at 48 h (P <0.05), but it was still higher than that of the control group at 96 h (P> 0.05) .Results showed that the resistance of spotted basket fish to low salt was relatively strong, By adjusting its own antioxidant enzyme activity to clear the body of excess free radicals, but relatively poor tolerance to freshwater environment.