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目的利用从Panc-02细胞株分离出的两种对γ-干扰素(IFN-γ)敏感性不同的肿瘤细胞,探讨肿瘤细胞IFN-γ敏感性对其在体试验中致瘤性的影响。方法IFN-γ刺激Panc-02细胞后,根据Ⅰ型表面组织相容抗原(MHCⅠ)的表达,利用流式细胞分类技术,分离出FS-3和PM-9细胞。将这些肿瘤细胞注射于小鼠的皮下,在21 d里比较肿瘤的生长情况。结果IFN-γ刺激FS-3,PM-9和Panc-02细胞后,FS-3的MHCⅠ表达最高,对IFN-γ的敏感性高；PM-9的MHCⅠ表达最低,对IFN-γ的敏感性低。把这3种肿瘤细胞注射于小鼠的皮下后,PM-9肿瘤的生长明显快于FS-3和Panc-02肿瘤。21 d后PM-9肿瘤的大小(139．1±30．2)mm~2明显大于FS-3和Panc-02肿瘤[(0．8±1．6),(14．3±6．7)mm~2,P＜0．01]。注射PM-9细胞的小鼠全部形成肿瘤(n=24,100%),而注射FS-3或Panc-2细胞的小鼠只有部分形成肿瘤(n=12,30%；n=24,75%；P＜0．05)。结论肿瘤细胞对IFN-γ敏感性影响其在体环境中的致瘤性。
OBJECTIVE: To determine the tumorigenicity of IFN-γ in tumor cells by using two tumor cells with different sensitivity to IFN-γ isolated from Panc-02 cell line. Methods After stimulating Panc-02 cells with IFN-γ, FS-3 and PM-9 cells were isolated by flow cytometry based on the expression of type Ⅰ surface antigen (MHCⅠ). These tumor cells were injected subcutaneously in mice and the tumor growth was compared over 21 days. Results FS-3 had the highest expression of MHC Ⅰ and high sensitivity to IFN-γ after IFN-γ stimulated FS-3, PM-9 and Panc-02 cells; PM-9 had the lowest expression of MHCⅠ and its sensitivity to IFN- Sex is low. PM-9 tumors grew significantly faster than FS-3 and Panc-02 tumors after injecting these 3 tumor cells subcutaneously in mice. After 21 days, the size of PM-9 tumors (139.1 ± 30.2) mm ~ 2 was significantly larger than that of FS-3 and Panc-02 tumors [(0.8 ± 1.6), (14.3 ± 6.7 ) mm ~ 2, P <0.01]. Mice injected with PM-9 cells all formed tumors (n = 24, 100%) whereas mice injected with FS-3 or Panc-2 cells only partially formed tumors (n = 12,30%; n = 24,75%; P <0.05). Conclusion The sensitivity of tumor cells to IFN-γ affects its tumorigenicity in vivo.