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目的建立体外子宫内膜培养模型,观察不同浓度瘦素(即leptin)对与着床密切相关的细胞间粘附分子-1(Intercellular Adhesion Molecule-1,即ICAM-1)、金属蛋白酶9(Matrix Metalloproteinase9,即MMP9)的影响,进而探讨leptin对胚胎早期着床的的调节作用。方法采用免疫细胞化学方法检测子宫内膜中ICAM-1和MMP9的表达;采用ELISA方法分别检测经leptin干预培养模型后培养液中ICAM-1和MMP9的表达。结果免疫细胞化学方法:ICAM-1主要在腺体细胞中有表达,基质细胞仅有少量的表达,而MMP9在子宫内膜基质细胞和腺体细胞中均有表达;ELISA结果显示:与对照组相比,ICAM-1经100ng/ml的leptin干预后表达上调(P<0.05),而经1ng/ml、10ng/ml的leptin干预后表达无显著性差异(P>0.05);MMP9分别经1ng/ml、10ng/ml、100ng/ml的leptin干预后表达上调(P<0.05)。结论一定量的leptin可以增加体外培养模型中ICAM-1和MMP9表达。
Objective To establish an endometrial culture model in vitro and observe the effect of leptin at different concentrations on the expression of Intercellular Adhesion Molecule-1 (ICAM-1), Matrix Metalloproteinase 9 Metalloproteinase9, namely MMP9), and then investigate the regulation of leptin on early embryo implantation. Methods The expression of ICAM-1 and MMP9 in endometrium was detected by immunocytochemistry. The expression of ICAM-1 and MMP9 in culture medium was detected by ELISA after leptin intervention. Results The results of immunocytochemistry showed that ICAM-1 was mainly expressed in glandular cells and only a small amount of stromal cells were expressed, while MMP9 was expressed in stromal cells and glandular cells of endometrium. ELISA results showed that ICAM- ICAM-1 was up-regulated after treatment with 100ng / ml leptin (P <0.05), but no significant difference was found when leptin was treated with 1ng / ml or 10ng / ml leptin (P> 0.05) / ml, 10ng / ml, 100ng / ml leptin after intervention (P <0.05). Conclusion A certain amount of leptin can increase the expression of ICAM-1 and MMP9 in vitro.