论文部分内容阅读
目的探讨肿瘤坏死因子基因6个SNPs位点多态性与河南汉族原发性高血压(EH)的相关性。方法采用限制性扩增片段长度多态性(PCR-RFLP)的方法检测TNF-α启动区基因-238G>A、-308G>A、-850C>T、-857C>T、-863C>A及TNF-β第一内含子基因+252G>A多态性,并运用卡方检验探讨各SNP位点多态性与EH的相关性。结果 TNF-α-308G>A位点基因型分布和等位基因频率在人群中存在差异显著性(χ2=6.407,P=0.041;χ2=4.781,P=0.029),EH组中,TNF-α-308G>A中基因型GA+AA人群的收缩压(SBP)、舒张压(DBP)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、尿酸(UA)及空腹血糖(FPG)含量明显高于GG基因型人群(P<0.05);TNF-α基因-238G>A、-850C>T、-857C>T、-863C>A及TNF-β基因+252G>A多态性各基因型及等位基因频率在EH组和对照组的分布差异无统计学意义。结论 TNF-α基因-308G>A位点基因多态性与河南汉族原发性高血压的发病相关。TNF-α-238G>A、-850C>T、-857C>T、-863C>A及TNF-β+252G>A位点基因多态性与河南汉族原发性高血压无关联。
Objective To investigate the association of polymorphisms of 6 SNPs in tumor necrosis factor gene with essential hypertension (EH) in Henan Han nationality. Methods The gene sequences of -238G> A, -308G> A, -850C> T, -857C> T and -863C> A of TNF-α promoter region were detected by PCR-RFLP TNF-β first intron gene + 252G> A polymorphism, and the use of chi-square test SNP locus polymorphism and EH correlation. Results The genotype distribution and allele frequency of TNF-α-308G> A locus were significantly different in the population (χ2 = 6.407, P = 0.041; χ2 = 4.781, P = 0.029) SBP, DBP, HDL, LDL, UA and FPG in GA + AA genotype of -308G> (P <0.05); TNF-α gene -238G> A, -850C> T, -857C> T, -863C> A and TNF-β gene + 252G> A polymorphism There was no significant difference in the distribution of genotype and allele between EH group and control group. Conclusion The polymorphism of -308G> A TNF-α gene is associated with the incidence of essential hypertension in Henan Han. The polymorphisms of TNF-α-238G> A, -850C> T, -857C> T, -863C> A and TNF-β + 252G> A were not associated with primary hypertension in Henan Han.