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DNA疫苗能够诱导机体产生特异的细胞免疫和体液免疫反应,在肿瘤和感染性疾病的疫苗开发中显示出巨大的潜能。以HIV-1核心蛋白P24为抗原基因,构建pVAX1-p24 DNA,经Western blotting和动物活体成像检测证明,pVAX1 DNA携带的外源基因可以在293T细胞和小鼠肌肉组织有效表达。采用不同的免疫策略免疫BALB/c小鼠(DNA/DNA,DNA/Protein),实验结果表明:pVAX1-p24单独免疫BALB/c小鼠,可诱导明显的体液免疫及细胞免疫反应;pVAX1-p24与P24蛋白联合免疫诱导的体液免疫反应高于pVAX1-p24单独免疫,所获得的抗体滴度是单独免疫的7.3~8.0倍,但细胞免疫反应则不及单独免疫组。研究结果表明采取不同的免疫策略可以诱导产生不同的免疫反应,根据具体情况调整免疫策略将获得更好的免疫效果。这些研究为艾滋病疫苗的研发提供了实验依据。
DNA vaccines can induce the body to produce specific cellular and humoral immune responses, showing great potential in the development of vaccines for tumors and infectious diseases. PVAX1-p24 DNA was constructed by using HIV-1 core protein P24 as an antigen gene. Western blotting and animal biopsy showed that the exogenous gene carried by pVAX1 DNA was efficiently expressed in 293T cells and mouse muscle tissues. BALB / c mice (DNA / DNA, DNA / Protein) were immunized with different immunization strategies. The results showed that: BALB / c mice immunized with pVAX1-p24 alone induced significant humoral and cellular immune responses; pVAX1-p24 The humoral immune response induced by co-immunization with P24 protein was higher than that of pVAX1-p24 alone, and the antibody titers obtained were 7.3- to 8.0-fold higher than that of immunization alone, but the cellular immune response was less than that of the immunized group alone. The results show that adopting different immunization strategies can induce different immune responses and adjusting immunization strategies according to specific conditions will achieve better immune effects. These studies provide experimental evidence for the development of AIDS vaccines.