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Mary E.Morton等最近利用细胞培养、免疫亲和性纯化分离技术以及电生理手段,证实抗二氢吡啶复合物α_1-亚单位的单克隆抗体(mAb 1A)对BC3H1肌细胞慢钙通道的I_(Ca~(2+))可产生不可逆性抑制。电压依从性钙通道在多种细胞的生理活动中起关键性作用。BC3H1肌细胞膜上存在两种钙通道,一种是高阈值的慢钙通道;另一种为低阈值的快钙通道,前者在肌细胞中占优势,并对二氢吡啶(DHP)敏感。DHP结合于膜蛋白,形成DHP复合物。某些实验室的工作曾认为,该复合物的成份是一大分子糖蛋白,其凝胶电泳行为受硫化物浓度的影响。最近有人发现,复合物中的一种新成份,称之为α_1-亚单位,其分子量
Mary E. Morton and other recently using cell culture, immunoaffinity purification techniques and electrophysiological methods, confirmed anti-dihydropyridine complex α_1-subunit monoclonal antibody (mAb 1A) BC3H1 myoblasts slow I_ (Ca ~ (2+)) can produce irreversible inhibition. Voltage-dependent calcium channels play a key role in the physiological activities of many types of cells. There are two types of calcium channels on the membrane of BC3H1 muscle, one is a high-threshold slow calcium channel and the other is a low-threshold fast calcium channel. The former predominates in muscle cells and is sensitive to dihydropyridine (DHP). DHP binds to membrane proteins to form DHP complexes. The work of some laboratories has considered that the composition of the complex is a macromolecule glycoprotein whose gel electrophoresis behavior is affected by the concentration of sulfide. Recently, it has been found that a new component of the complex, called the α_1-subunit, has a molecular weight