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目的 本实验用亲合免疫组织化学技术 ,研究小鼠耳蜗内nNOS与eNOS的表达。方法 4%多聚甲醛心脏灌注固定后 ,取耳蜗 ,经脱钙 ,作 10 μm厚冰冻切片 ,进行nNOS和eNOS免疫组织化学染色。 结果 小鼠耳蜗内、外毛细胞、内外柱细胞、螺旋神经节细胞nNOS、eNOS的表达呈强阳性。血管纹的基底和中间细胞、螺旋突起、螺旋韧带细胞处有阳性nNOS、eNOS的表达。耳蜗小球的内皮细胞无nNOS的表达 ,但eNOS的表达呈阳性。结论 由nNOS和eNOS合成的NO在维持耳蜗正常神经传导及耳蜗正常血液供应中起着重要作用
Objective To study the expression of nNOS and eNOS in the mouse cochlea by affinity immunohistochemistry. Methods After 4% paraformaldehyde perfusion, the cochleas were removed and decalcified to make frozen section of 10 μm for nNOS and eNOS immunohistochemical staining. Results The expression of nNOS and eNOS in inner and outer hair cells, inner and outer column cells and spiral ganglion cells in mice was strongly positive. Vascular pattern of basal and intermediate cells, spiral protrusions, spiral ligament cells have positive nNOS, eNOS expression. Endothelial cells of cochlear globules showed no nNOS expression, but eNOS expression was positive. Conclusions NO synthesized by nNOS and eNOS plays an important role in maintaining normal cochlear nerve conduction and normal cochlear blood supply