在原位杂交研究的基础上,本文对慢性乙肝患者胞浆型HBV DNA及肝内其它乙肝指标进行综合分析。发现胞浆型HBV DNA可分3种类型:①肝内同时有核衣壳蛋白(HBcAg)与包膜蛋白(HBsAg)表达,此与HBV复制的产物表达特征一致;②肝内仅有HBsAg表达,此与HBVDNA和宿主基因组整合后的表达特点相似;③肝内HBsAg、HBcAg表达均缺如,可能代表一种HBV非复制状态。由此表明在HBV非复制期,出现胞浆HBV DNA杂交信号可能系DNA RNA杂交体所致。 On the basis of in situ hybridization studies, this paper comprehensively analyzed cytosolic HBV DNA and other intrahepatic HBV markers in patients with chronic hepatitis B. It was found that cytoplasmic HBV DNA can be divided into three types: (1) the expression of both HBcAg and HBsAg in the liver is consistent with the expression characteristics of the HBV replication; (2) only the HBsAg expression in the liver , Which is similar to the expression characteristics of HBVDNA and host genome. ③ The absence of HBsAg and HBcAg expression in the liver may represent a non-HBV replication state. This shows that in the HBV non-replication phase, the emergence of cytoplasmic HBV DNA hybridization signal may be caused by DNA RNA hybrids.