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目的 探讨外周血多形核白细胞化学发光 (Polymorphonuclear Chemilluminescence ,PMN CL)及细胞因子活性改变与原发性肝癌的发病机制关系。方法 采用液体闪烁技术 (LSC)体外激活PMN生成、释放过氧化阴离子 ,诱导吞噬作用产生化学发光 ,并应用ELISA及RIA检测相关指标IL 2、sIL 2R、IL 8、TNF、SOD ,并以健康献血员为对照。结果 体内激活明显 ,基础CL升高 (P <0 .0 5 ) ,最大峰值低下 ,峰时延迟 (P <0 .0 1)。相关指标IL 9、sIL 2R、TNF、SOD显著高于对照组 (P <0 .0 1) ,IL 2低于对照组 (P <0 .0 5 )。结论 原发性肝癌患者外周血PMN化学发光变化 ,客观反应了细胞免疫功能水平 ,以及超氧离子的生成、释放与损伤状态。对深入研究原发性肝癌的发病机理具有重要意义。
Objective To investigate the relationship between changes of polymorphonuclear leukocyte chemiluminescence (PMN CL) and cytokines in peripheral blood and the pathogenesis of primary liver cancer. Methods PMNs were activated by liquid scintillation technique (LSC) to produce and release peroxides and induce phagocytosis to produce chemiluminescence. IL-2, sIL 2R, IL 8, TNF and SOD were detected by ELISA and RIA. Members as a control. Results In vivo activation was obvious, basal CL was increased (P <0. 05), the maximum peak was low and the peak was delayed (P <0.01). IL-9, sIL 2R, TNF and SOD were significantly higher than those in the control group (P <0.01), IL 2 was lower than the control group (P <0.05). Conclusion The changes of PMN chemiluminescence in peripheral blood of patients with primary hepatic carcinoma objectively reflect the level of cellular immunity and the generation, release and damage of superoxide ion. It is of great significance to further study the pathogenesis of primary liver cancer.