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用稻曲病菌分生孢子悬浮液对抽穗期水稻进行接种,观察分生孢子及其侵染途径。结果发现,分生孢子在颖壳表面可萌发形成菌丝,在颖口内侧可以见到菌丝伸向谷颖内部,可能为分生孢子直接侵染稻穗提供了一定证据。对抗、感品种谷粒的组织化学研究结果表明:抗病品种的颖壳中含有大量的木质素,感病品种中的木质素较少;在抗病品种谷粒的颖壳中的红色荧光物质远远多于感病品种;在抗病品种谷粒表皮的胚乳细胞中也含有多酚类物质,这层细胞较正常细胞大,在感病品种中没有发现。稻曲球切片的紫外光观察可发现每一个稻曲球中存在 6个“蝴蝶”型荧光结构,染色剂染色后观察证明该结构不含过氧化物酶、单宁类物质、木栓质和木质素。
Inoculation of rice at heading stage with conidial suspension of Ustilaginoidea infection and observation of conidia and their infection pathways. The results showed that the conidiospores germinated on the surface of the glume shell, and mycelium could be seen inside the glume wall. It may provide some evidences that the conidia directly invaded the panicle. The results of histochemical studies on the resistant and susceptible grain showed that the glume of resistant cultivars contained a large amount of lignin, while the less susceptible cultivars contained less lignin. In the glume of the resistant cultivars, the red fluorescent material Far more than the susceptible varieties; the endosperm cells in the grain epidermis of the resistant varieties also contain polyphenols, which are larger than normal cells and not found in susceptible varieties. The results showed that there were 6 “butterfly” fluorescent structures in each of the inflorescences of rice. UV-visible observation of the stain showed that the structure was free of peroxidase, tannin, cork and Lignin.