论文部分内容阅读
目的:探讨乙烯利对青春期大鼠睾丸组织病理及生精细胞凋亡的影响。方法:青春期雄性25日龄SD大鼠,分别以乙烯利浓度为2000 mg/kg、1000 mg/kg、500 mg/kg和生理盐水连续灌胃14和21天,分别取睾丸固定、包埋,以HE染色、末端转移酶标记技术(TUNE法)光镜观察睾丸的组织形态学变化、检测生精细胞凋亡情况。结果:低剂量组较对照组相比生精小管萎缩,生精细胞排列紊乱;中剂量和高剂量组较低剂量组相比,生精小管更加萎缩,生精细胞排列明显紊乱;接触乙烯利14天后高剂量组生精细胞凋亡指数与低剂量、对照组相比具有显著统计学差异(P<0.01);高剂量与中剂量组相比无统计学差异(P>0.05);中剂量和低剂量与对照组相比有显著统计学差异(P<0.01);接触乙烯利21天后各组间比较均具有显著性差异(P<0.01)。结论:乙烯利可导致大鼠生精细胞凋亡增加,生精能力下降,这可能是导致青年不育的原因之一。
Objective: To investigate the effects of ethephon on testicular histopathology and apoptosis of spermatogenic cells in adolescent rats. METHODS: Adolescent male 25-day-old Sprague-Dawley rats were treated with ethephon 2000 mg / kg, 1000 mg / kg, 500 mg / kg and saline respectively for 14 and 21 days. The testis was fixed, embedded, The morphological changes of testes were observed by HE staining and TUNEL method. The apoptosis of spermatogenic cells was detected. Results: Compared with the control group, the spermatogenic atrophy and the spermatogenic cells were disordered in the low-dose group. Compared with the low-dose group, the seminiferous tubules were more constricted and the spermatogenic cells were obviously disordered compared with the low-dose group. After 14 days, the apoptotic index of spermatogenic cells in high dose group was significantly lower than that in control group (P <0.01); there was no significant difference between high dose group and middle dose group (P> 0.05) (P <0.01). There was a significant difference between the two groups after exposure to ethephon for 21 days (P <0.01). CONCLUSION: Ethephon can induce the apoptosis of spermatogenic cells in rats and decrease the spermatogenic capacity, which may be one of the causes of infertility in young people.