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克隆编码人ＣＤ4 0Ｌ全长ｃＤＮＡ的分子。用淋巴细胞分离液分离健康人淋巴细胞并用ＰＨＡ激活 ,2 4ｈ后抽提ｍＲＮＡ ,以ＲＴ ＰＣＲ方法克隆编码人ＣＤ4 0Ｌ基因全长ｃＤＮＡ ,序列测定证实其正确性。结果表明 ,ＲＴ ＰＣＲ产物经直接测序证实人ＣＤ4 0Ｌ基因全序列克隆成功
Clone a molecule encoding human CD4 0L full-length cDNA. Human lymphocytes were isolated from lymphocytes and activated with PHA. MRNA was extracted after 24 hours. The full length cDNA of human CD4 O gene was cloned by RT PCR. The sequence was used to confirm the correctness of the results. The results showed that RT-PCR products were confirmed by direct sequencing of human CD4 0L gene cloned successfully