因取材困难易污染等原因欲测定人内耳外淋巴液中的化学成分很困难。本文作者们在18例尸体(50～70岁,多为心力衰竭而死亡)取外淋巴液进行蛋白分析,每例尸体取到5～7μ1外淋巴液,同时比较脑脊液和血清的测定结果。为精确测定外淋巴液的蛋白分布,采用了琼脂糖电泳、免疫组织固相法、交叉免疫电泳、十二烷基硫酸钠聚丙酰胺凝胶电泳(SDS-PAGE)等方法, It is difficult to determine the chemical composition of the human inner and outer lymph of the inner ear due to difficulties in material drawing and easy contamination. The authors performed extracellular lymphadenectomy in 18 corpses (50-70 years of age, mostly deaths from heart failure). Peripheral lymphatic fluid was obtained from 5 to 7 μl for each case, and cerebrospinal fluid and serum were also compared. In order to accurately determine the protein distribution of perilymph, we used agarose gel electrophoresis, immunohistochemistry, cross-immunoelectrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)