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目的探讨延髓内脏带(MVZ)与下丘脑室旁核(PVN)和视上核(SON)之间是否存在往返渗透压投射通路。方法通过给予大鼠饮用3%氯化钠的方法制作高渗刺激模型,并用WGA-HRP逆行追踪、抗Fos、抗酪氨酸羟化酶(TH)或加压素(VP)及胶质纤维酸性蛋白(GFAP)免疫组织化学相结合的四重标记方法,观察MVZ、PVN和SON中WGA-HRP、Fos、TH、VP和GFAP阳性分布及表达状况。结果高渗刺激后MVZ、PVN和SON内Fos阳性细胞明显增多;GFAP阳性结构也明显增多,其分布与Fos阳性细胞分布基本一致,表现为胞体肥大、突起粗长。星形胶质细胞(AST)紧密包绕在神经元周围形成神经元-AST复合体(N-ASC)。结论神经元和AST以N-ASC的形式共同参与渗透压调节反应,体内存在MVZ和SON或PVN之间往返的渗透压调节通路。
Objective To investigate whether there is a reciprocating osmotic pressure projection pathway between medullary visceral zone (MVZ) and hypothalamic paraventricular nucleus (PVN) and supraoptic nucleus (SON). Methods Hypertonic stimulation model was established by administering 3% sodium chloride to rats. WGA-HRP retrograde tracing, anti-Fos, anti-tyrosine hydroxylase (TH), vasopressin (VP) and glial fibrils The positive expression and distribution of WGA-HRP, Fos, TH, VP and GFAP in MVZ, PVN and SON were observed by the quadruple-labeling method combined with immunohistochemistry and acidic protein (GFAP) immunohistochemistry. Results After hypertonic stimulation, the number of Fos positive cells in MVZ, PVN and SON increased obviously. The positive expression of GFAP also increased obviously. The distribution of Fos positive cells was basically consistent with the distribution of Fos positive cells. Astrocytes (AST) tightly wrap around neurons to form neuronal-AST complex (N-ASC). Conclusion Neurons and AST participate in the osmoregulation reaction in the form of N-ASC, and there is an osmotic pressure regulating pathway between MVZ and SON or PVN in vivo.